Medical Definition of Dna hybridisation

1. The process of joining two complementary strands of DNA or one each of DNA and RNA to form a double-stranded molecule. Technique in which single stranded nucleic acids are allowed to interact so that complexes or hybrids, are formed by molecules with sufficiently similar, complementary sequences. By this means the degree of sequence identity can be assessed and specific sequences detected. The hybridisation can be carried out in solution or with one component immobilised on a gel or, most commonly, nitrocellulose paper. Hybrids are detected by various means: visualisation in the electron microscope, by radioactively labelling one component and removing noncomplexed DNA or by washing or digestion with an enzyme that attacks single stranded nucleic acids and finally estimating the radioactivity bound. Hybridisations are done in all combinations: DNA DNA (DNA can be rendered single stranded by heat denaturation), DNA RNA or RNA RNA. In situ hybridisations involve hybridising a labelled nucleic acid (often labelled with a fluorescent dye) to suitably prepared cells or histological sections. This is used particularly to look for specific transcription or localisation of genes to specific chromosomes (FISH analysis). The mating of individuals from different species or sub-species. (13 Oct 1997)



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Lexicographical Neighbors of Dna Hybridisation

DNA entry nuclease
DNA excision
DNA fingerprint
DNA fingerprinting
DNA footprinting
DNA fragmentation
DNA gap
DNA gene
DNA glycosylase
DNA gyrase
DNA helicase
DNA helicases
DNA helix
DNA homology
DNA hybridisation
DNA hybridization
DNA insertion elements
DNA iteron
DNA ladder
DNA ladders
DNA library
DNA ligase
DNA ligases
DNA ligation
DNA markers
DNA melting
DNA methylation
DNA microarray
DNA microarrays

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