Medical Definition of Isoelectric focusing
1.
Electrophoresis in a stabilised pH gradient. High resolution method for separating molecules, especially proteins, that carry both positive and negative charges. Molecules migrate to the pH corresponding to their isoelectric point. The gradient is produced by electrophoresis of amphiphiles, heterogenous molecules giving a continuum of isoelectric points. Resolution is determined by the number of amphiphile species and the evenness of distribution of their isoelectric points.
This entry appears with permission from the Dictionary of Cell and Molecular Biology
(11 Mar 2008)
Lexicographical Neighbors of Isoelectric Focusing
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