Definition of Indirect immunofluorescence

1. Noun. A method of using fluorescence microscopy to detect the presence of an antigen indirectly.

Generic synonyms: Fluorescence Microscopy

Medical Definition of Indirect immunofluorescence

1. A method of immunofluorescence staining in which the first antibody, that is directed against the antigen to be localised, is used unlabelled and the location of the first antibody is then detected by use of a fluorescently labelled antiIgG (against IgGs of the species in which the first antibody was raised). The advantage is that there is some amplification and a well characterised goat antirabbit IgG antibody can, for example: be used against a scarce specific antibody raised in rabbits. The same technique can be used for ultrastructural localisation of the first antibody by substituting peroxidase or gold labelled second antibody. This entry appears with permission from the Dictionary of Cell and Molecular Biology (11 Mar 2008)

Indirect Immunofluorescence Pictures

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Lexicographical Neighbors of Indirect Immunofluorescence

indirect Coombs' test
indirect agglutination
indirect antonym
indirect bilirubin
indirect correlation
indirect discourse
indirect diuretic
indirect evidence
indirect expression
indirect fire
indirect fluorescent antibody
indirect fracture
indirect free kick
indirect haemagglutination test
indirect immunofluorescence (current term)
indirect lead
indirect life cycle
indirect lighting
indirect liquefaction
indirect maternal death
indirect nuclear division
indirect object
indirect objects
indirect ophthalmoscope
indirect ophthalmoscopy
indirect placentography
indirect pulp capping
indirect pupillary reaction

Literary usage of Indirect immunofluorescence

Below you will find example usage of this term as found in modern and/or classical literature:

1. Science by American Association for the Advancement of Science (1883)
"Antibodies to early antigens were detected by indirect immunofluorescence in serums of many patients with infectious mononucleosis, Burkitt's lymphoma, ..."

2. Diarrhea and Malnutrition: Interactions, Mechanisms, and Interventions by Lincoln C. Chen, Nevin S. Scrimshaw (1983)
"... of production of antibody and T-cell-mediated immune response in the systemic and secretory sites. T-Lymphocytes, indirect immunofluorescence with ..."

3. Proceedings of the Society for Experimenta (1903)
"PCP, IgG, and IgM in rat kidney were evaluated by indirect immunofluorescence, as described in detail elsewhere (5). Briefly, tissue was quick frozen and ..."

4. A Lifelong Passion: Nicholas and Alexandra: Their Own Story by Andrei Maylunas (2005)
"Cell phenotypes were evaluated by a panel of monoclonal antibodies by indirect Immunofluorescence on cell sediments. Cytotoxic activity was assessed by ..."

5. Manual of Microbiologic Monitoring of Laboratory Animals edited by Kim Waggie (1994)
"Also, antibody had been demonstrated (indirect immunofluorescence) in the sera of barrier-maintained colonies of mice, rats, and rabbits. ..."

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