Lexic.us

Definition of Indirect immunofluorescence

1. Noun. A method of using fluorescence microscopy to detect the presence of an antigen indirectly.

Medical Definition of Indirect immunofluorescence

1. A method of immunofluorescence staining in which the first antibody, that is directed against the antigen to be localised, is used unlabelled and the location of the first antibody is then detected by use of a fluorescently labelled antiIgG (against IgGs of the species in which the first antibody was raised). The advantage is that there is some amplification and a well characterised goat antirabbit IgG antibody can, for example: be used against a scarce specific antibody raised in rabbits. The same technique can be used for ultrastructural localisation of the first antibody by substituting peroxidase or gold labelled second antibody. This entry appears with permission from the Dictionary of Cell and Molecular Biology (11 Mar 2008)

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Lexicographical Neighbors of Indirect Immunofluorescence

Literary usage of Indirect immunofluorescence

Below you will find example usage of this term as found in modern and/or classical literature:

1. Science by American Association for the Advancement of Science (1883)
"Antibodies to early antigens were detected by indirect immunofluorescence in serums of many patients with infectious mononucleosis, Burkitt's lymphoma, ..."

2. Diarrhea and Malnutrition: Interactions, Mechanisms, and Interventions by Lincoln C. Chen, Nevin S. Scrimshaw (1983)
"... of production of antibody and T-cell-mediated immune response in the systemic and secretory sites. T-Lymphocytes, indirect immunofluorescence with ..."

3. Proceedings of the Society for Experimenta (1903)
"PCP, IgG, and IgM in rat kidney were evaluated by indirect immunofluorescence, as described in detail elsewhere (5). Briefly, tissue was quick frozen and ..."

4. A Lifelong Passion: Nicholas and Alexandra: Their Own Story by Andrei Maylunas (2005)
"Cell phenotypes were evaluated by a panel of monoclonal antibodies by indirect Immunofluorescence on cell sediments. Cytotoxic activity was assessed by ..."

5. Manual of Microbiologic Monitoring of Laboratory Animals edited by Kim Waggie (1994)
"Also, antibody had been demonstrated (indirect immunofluorescence) in the sera of barrier-maintained colonies of mice, rats, and rabbits. ..."

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