Definition of Indirect immunofluorescence
1. Noun. A method of using fluorescence microscopy to detect the presence of an antigen indirectly.
Medical Definition of Indirect immunofluorescence
1.
A method of immunofluorescence staining in which the first antibody, that is directed against the antigen to be localised, is used unlabelled and the location of the first antibody is then detected by use of a fluorescently labelled antiIgG (against IgGs of the species in which the first antibody was raised). The advantage is that there is some amplification and a well characterised goat antirabbit IgG antibody can, for example: be used against a scarce specific antibody raised in rabbits. The same technique can be used for ultrastructural localisation of the first antibody by substituting peroxidase or gold labelled second antibody.
This entry appears with permission from the Dictionary of Cell and Molecular Biology
(11 Mar 2008)
Lexicographical Neighbors of Indirect Immunofluorescence
Other Resources:
