Definition of Photoaffinity labelling
1. Noun. (alternative spelling of photoaffinity labeling) ¹
¹ Source: wiktionary.com
Medical Definition of Photoaffinity labelling
A technique for covalently attaching a label or marker molecule onto another molecule such as a protein.
The label, which is often fluorescent or radioactive, contains a group that becomes chemically reactive when illuminated usually with ultraviolet light) and will form a covalent linkage with an appropriate group on the molecule to be labelled: proximity is essential. The most important class of photoreactive groups used are the aryl azides, which form short lived but highly reactive nitrenes when illuminated.
(31 Dec 1997)
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